Buffer: 20 mm Tris-HCl, 0.15 M NaCl, ph Water at room temperature. After attachment the pka is expected to be in the range of 10.5 to Median particle size of the cumulative volume distribution 3 Determined at 10% breakthrough with a residence time of 3 minutes (1.6 ml/min = 200 cm/h) in HiScreen columns. Characteristics of Capto Core 700 Matrix Size cut-off of outer layer pka of protonated octylamine 1 Functional group in the core Ionic capacity Average particle size (d 50v ) 2 Dynamic binding capacity 3 High flow agarose M r ~ CH 3 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 NH- 40 to 85 µmol (Cl - /ml resin) 90 µm ~13 mg ovalbumin/ml resin Maximum flow velocity cm/h ph stability, operational 5 3 to 13 ph stability, CIP 6 3 to 14 Operating temperature 4 C to 30 C Chemical stability All commonly used aqueous buffers 1 M sodium hydroxide 7 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol Avoid Oxidizing agents, anionic detergents Storage 4 C to 30 C in 20% ethanol 1 pka of protonated octylamine before attachment to the resin. Further characteristics of the chromatography resin are found in Table AG 5Ħ Table 3. Thus Capto Core 700 provides excellent productivity and process economy. The design of the bead, with a layer that prevents binding of the large target, allows for high resolution at high flow rates. The product is based on a high flow agarose base matrix, which gives good flow properties. Large targets are excluded from the beads, whereas smaller impurities can enter into the core where they are being captured by the ligands. Schematic representation of the principle for Capto Core 700. The octylamine ligand (see Table 3) is both hydrophobic and positively charged in order to interact strongly with most of the impurities over a wide range of ph and salt concentrations. A schematic representation of Capto Core 700 is given in Figure 3. The molecular size cut-off for proteins is approximately M r = which means that targets that are larger than this relative molecular mass will pass through the column in the flowthrough fraction.
This outer layer prevents large targets from binding to the ligands whereas smaller impurities can enter freely into the beads where they are being captured. The core is surrounded by a layer without ligands. Each bead has a core activated with octylamine ligands. Safety For use and handling of the products in a safe way, refer to the Safety Data Sheets AGĥ Properties of Capto Core 700 Capto Core 700 is based on the core bead concept. Intended use The products are intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes. 7 Storage Troubleshooting Ordering information Read these instructions carefully before using the products.
4 Cleaning-In-Place (CIP) Scaling up Adjusting pressure limits in chromatography system software. The columns are used in an optimal way with liquid chromatography systems such as ÄKTA.Ģ Table of Contents 1 Product description. HiScreen Capto Core 700 and HiTrap Capto Core 700 columns provide fast, reproducible, and easy separations in convenient formats.
Due to the core beads concept, Capto Core 700 offers high purity, improved process economy, and increased productivity compared to size exclusion chromatography in group separation mode by enabling: Up to 100 times higher load Significantly higher flow rates Straightforward optimization and scale up HiScreen Capto Core 700 (4.7 ml) and HiTrap Capto Core 700 (1 ml) are prepacked columns for optimization of methods and parameters, such as sample load and binding conditions, as well as small scale purifications. The layer prevents the large targets from entering into the beads whereas smaller proteins and impurities enter into the core where they bind to the hydrophobic and positively charged octylamine ligands. Each bead has a ligand-activated core and an outer layer without ligands. The product is based on the core bead concept. 1 Instructions AG HiScreen Capto Core 700 HiTrap Capto Core 700, 1 ml Capto Core 700 chromatography resin is aimed at intermediate purification and polishing of viruses and other large biomolecules in flow-through mode.
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